Constructing and transient expression of a gene cassette containing edible vaccine elements and shigellosis, anthrax and cholera recombinant antigens in tomato
Identifieur interne : 000E33 ( Main/Exploration ); précédent : 000E32; suivant : 000E34Constructing and transient expression of a gene cassette containing edible vaccine elements and shigellosis, anthrax and cholera recombinant antigens in tomato
Auteurs : Jafari Davod [Iran] ; Dehghan Nayeri Fatemeh [Iran] ; Hossein Honari [Iran] ; Ramin Hosseini [Iran]Source :
- Molecular Biology Reports [ 0301-4851 ] ; 2018.
Abstract
Url:
DOI: 10.1007/s11033-018-4385-3
PubMed: 30244396
PubMed Central: 7088786
Affiliations:
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Iran</nlm:aff><country xml:lang="fr">Iran</country><wicri:regionArea>Qazvin</wicri:regionArea></affiliation></author><author><name sortKey="Honari, Hossein" sort="Honari, Hossein" uniqKey="Honari H" first="Hossein" last="Honari">Hossein Honari</name><affiliation wicri:level="1"><nlm:aff id="Aff3">Faculty of Basic Science, Imam Hussein University, Tehran, Iran</nlm:aff><country xml:lang="fr">Iran</country><wicri:regionArea>Faculty of Basic Science, Imam Hussein University, Tehran</wicri:regionArea><wicri:noRegion>Tehran</wicri:noRegion></affiliation></author><author><name sortKey="Hosseini, Ramin" sort="Hosseini, Ramin" uniqKey="Hosseini R" first="Ramin" last="Hosseini">Ramin Hosseini</name><affiliation wicri:level="1"><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="ISNI">0000 0000 8608 1112</institution-id><institution-id institution-id-type="GRID">grid.411537.5</institution-id><institution>Biotechnology Department, Faculty of Agricultural and Natural Sciences,</institution><institution>Imam Khomeini International University (IKIU),</institution></institution-wrap>Qazvin, Iran</nlm:aff><country xml:lang="fr">Iran</country><wicri:regionArea>Qazvin</wicri:regionArea></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">30244396</idno><idno type="pmc">7088786</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7088786</idno><idno type="RBID">PMC:7088786</idno><idno type="doi">10.1007/s11033-018-4385-3</idno><date when="2018">2018</date><idno type="wicri:Area/Pmc/Corpus">000303</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000303</idno><idno type="wicri:Area/Pmc/Curation">000303</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000303</idno><idno type="wicri:Area/Pmc/Checkpoint">000340</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000340</idno><idno type="wicri:Area/Ncbi/Merge">000816</idno><idno type="wicri:Area/Ncbi/Curation">000816</idno><idno type="wicri:Area/Ncbi/Checkpoint">000816</idno><idno type="wicri:doubleKey">0301-4851:2018:Davod J:constructing:and:transient</idno><idno type="wicri:Area/Main/Merge">000E35</idno><idno type="wicri:Area/Main/Curation">000E33</idno><idno type="wicri:Area/Main/Exploration">000E33</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Constructing and transient expression of a gene cassette containing edible vaccine elements and shigellosis, anthrax and cholera recombinant antigens in tomato</title><author><name sortKey="Davod, Jafari" sort="Davod, Jafari" uniqKey="Davod J" first="Jafari" last="Davod">Jafari Davod</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0004 4911 7066</institution-id><institution-id institution-id-type="GRID">grid.411746.1</institution-id><institution>Medical Biotechnology Department, School of Allied Medicine,</institution><institution>Iran University of Medical Sciences,</institution></institution-wrap>Tehran, Iran</nlm:aff><country xml:lang="fr">Iran</country><wicri:regionArea>Tehran</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="ISNI">0000 0000 8608 1112</institution-id><institution-id institution-id-type="GRID">grid.411537.5</institution-id><institution>Biotechnology Department, Faculty of Agricultural and Natural Sciences,</institution><institution>Imam Khomeini International University (IKIU),</institution></institution-wrap>Qazvin, Iran</nlm:aff><country xml:lang="fr">Iran</country><wicri:regionArea>Qazvin</wicri:regionArea></affiliation></author><author><name sortKey="Fatemeh, Dehghan Nayeri" sort="Fatemeh, Dehghan Nayeri" uniqKey="Fatemeh D" first="Dehghan Nayeri" last="Fatemeh">Dehghan Nayeri Fatemeh</name><affiliation wicri:level="1"><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="ISNI">0000 0000 8608 1112</institution-id><institution-id institution-id-type="GRID">grid.411537.5</institution-id><institution>Biotechnology Department, Faculty of Agricultural and Natural Sciences,</institution><institution>Imam Khomeini International University (IKIU),</institution></institution-wrap>Qazvin, Iran</nlm:aff><country xml:lang="fr">Iran</country><wicri:regionArea>Qazvin</wicri:regionArea></affiliation></author><author><name sortKey="Honari, Hossein" sort="Honari, Hossein" uniqKey="Honari H" first="Hossein" last="Honari">Hossein Honari</name><affiliation wicri:level="1"><nlm:aff id="Aff3">Faculty of Basic Science, Imam Hussein University, Tehran, Iran</nlm:aff><country xml:lang="fr">Iran</country><wicri:regionArea>Faculty of Basic Science, Imam Hussein University, Tehran</wicri:regionArea><wicri:noRegion>Tehran</wicri:noRegion></affiliation></author><author><name sortKey="Hosseini, Ramin" sort="Hosseini, Ramin" uniqKey="Hosseini R" first="Ramin" last="Hosseini">Ramin Hosseini</name><affiliation wicri:level="1"><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="ISNI">0000 0000 8608 1112</institution-id><institution-id institution-id-type="GRID">grid.411537.5</institution-id><institution>Biotechnology Department, Faculty of Agricultural and Natural Sciences,</institution><institution>Imam Khomeini International University (IKIU),</institution></institution-wrap>Qazvin, Iran</nlm:aff><country xml:lang="fr">Iran</country><wicri:regionArea>Qazvin</wicri:regionArea></affiliation></author></analytic><series><title level="j">Molecular Biology Reports</title><idno type="ISSN">0301-4851</idno><idno type="eISSN">1573-4978</idno><imprint><date when="2018">2018</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p id="Par1"><italic>Shigella dysenteriae</italic> causing shigellosis is one of the diseases that threaten the health of human society in the developing countries. In Shigella, <italic>IpaD</italic> gene is one of the key pathogenic genes causing strong mucosal immune system reactions. Anthrax disease is caused by <italic>Bacillus anthracis</italic>. PA protective antigen is one of the subunits in anthrax toxin complex responsible for the transfer of other subunits into the cytosol of host cells. The 20 kDa subunit of PA (PA20) has the property of immunogenicity. CTxB or B subunit of <italic>Vibrio cholerae</italic> toxin (CT) is a non-toxic protein and has the function to transfer toxic subunit into cytosol of the host cells by binding to GM1 receptor. The aim of this study was to fuse <italic>PA20, ipaD</italic> and <italic>CTxB</italic> and transform tomato plants by this cassette in order to produce an oral vaccine against shigellosis, anthrax and cholera. CTxB was used for these two antigens as an immune adjuvant. <italic>IpaD</italic> and <italic>PA20</italic> genes were cloned in pBI121 containing the <italic>CTxB</italic> gene and Extensin signal peptide. In order to evaluate the transient expression of Shigellosis, Anthrax and Cholera antigens, agro-infiltrated tomato tissues were inoculated with <italic>Agrobacterium tumefaciens</italic> containing the gene cassette. Cloning was confirmed by PCR, enzymatic digestion and sequencing techniques. Expression of the antigens was examined by SDS-PAGE, dot blot and ELISA. Maturate green fruits demonstrated the highest expression of the recombinant proteins. The first phase of this study was carried out for cloning and expressing of CtxB, ipaD and PA20 antigens in tomato. In the next phase, we aim to analyze the immunogenicity of this vaccine candidate in laboratory animals.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Gangarosa, Ej" uniqKey="Gangarosa E">EJ Gangarosa</name></author><author><name sortKey="Perera, Dr" uniqKey="Perera D">DR Perera</name></author><author><name sortKey="Mata, Lj" uniqKey="Mata L">LJ Mata</name></author><author><name sortKey="Morris, Cm" uniqKey="Morris C">CM Morris</name></author><author><name sortKey="Guzman, G" uniqKey="Guzman G">G Guzman</name></author><author><name sortKey="Reller, Lb" uniqKey="Reller L">LB Reller</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sansonetti, Pj" uniqKey="Sansonetti P">PJ Sansonetti</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sasakawa, C" uniqKey="Sasakawa C">C Sasakawa</name></author><author><name sortKey="Kamata, K" uniqKey="Kamata K">K 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Pogrebnyak</name></author><author><name sortKey="Golovkin, M" uniqKey="Golovkin M">M Golovkin</name></author><author><name sortKey="Andrianov, V" uniqKey="Andrianov V">V Andrianov</name></author><author><name sortKey="Spitsin, S" uniqKey="Spitsin S">S Spitsin</name></author><author><name sortKey="Smirnov, Y" uniqKey="Smirnov Y">Y Smirnov</name></author><author><name sortKey="Egolf, R" uniqKey="Egolf R">R Egolf</name></author><author><name sortKey="Koprowski, H" uniqKey="Koprowski H">H Koprowski</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chowdhury, K" uniqKey="Chowdhury K">K Chowdhury</name></author><author><name sortKey="Bagasra, O" uniqKey="Bagasra O">O Bagasra</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sala, F" uniqKey="Sala F">F Sala</name></author><author><name sortKey="Rigano, Mm" uniqKey="Rigano M">MM Rigano</name></author><author><name sortKey="Barbante, A" uniqKey="Barbante A">A Barbante</name></author><author><name sortKey="Basso, B" uniqKey="Basso B">B Basso</name></author><author><name sortKey="Walmsley, Am" uniqKey="Walmsley A">AM Walmsley</name></author><author><name sortKey="Castiglione, S" uniqKey="Castiglione S">S Castiglione</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Jafari, D" uniqKey="Jafari D">D Jafari</name></author><author><name sortKey="Dehghan, Nf" uniqKey="Dehghan N">NF Dehghan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Jafari, D" uniqKey="Jafari D">D Jafari</name></author><author><name sortKey="Dehghan Nayeri, F" uniqKey="Dehghan Nayeri F">F Dehghan Nayeri</name></author><author><name sortKey="Honari, H" uniqKey="Honari H">H Honari</name></author><author><name sortKey="Hoseini, R" uniqKey="Hoseini R">R Hoseini</name></author><author><name sortKey="Jafari, R" uniqKey="Jafari R">R Jafari</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>Iran</li></country></list><tree><country name="Iran"><noRegion><name sortKey="Davod, Jafari" sort="Davod, Jafari" uniqKey="Davod J" first="Jafari" last="Davod">Jafari Davod</name></noRegion><name sortKey="Davod, Jafari" sort="Davod, Jafari" uniqKey="Davod J" first="Jafari" last="Davod">Jafari Davod</name><name sortKey="Fatemeh, Dehghan Nayeri" sort="Fatemeh, Dehghan Nayeri" uniqKey="Fatemeh D" first="Dehghan Nayeri" last="Fatemeh">Dehghan Nayeri Fatemeh</name><name sortKey="Honari, Hossein" sort="Honari, Hossein" uniqKey="Honari H" first="Hossein" last="Honari">Hossein Honari</name><name sortKey="Hosseini, Ramin" sort="Hosseini, Ramin" uniqKey="Hosseini R" first="Ramin" last="Hosseini">Ramin Hosseini</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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